MTT cell line, compounds 6 and 9

MTT cytotoxicity assay

In
vitro cytotoxicity test is mainly performed to screen
potentially toxic compounds that affect basic cellular functions 26. The
molecular cancer cell lines profiling is also essential for the development of
new anticancer drugs and for understanding the mechanism of action and the
patterns involved in cell resistance to chemotherapeutics already used in the
treatment of cancer. Moreover, cancer cell lines can be a powerful tool for the
identification of genes alterations or pathways related to cancer and for the
discovery of putative drug targets 27.

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In MCF-7 breast cancer cell line study,
compounds 6 (3,6,7- trihydroxy-2-(3-methoxyphenyl)-4H-chromen-4-one) and 9
(isopropyl linoleate) exhibited good cytotoxic activity with the IC50
value of 83.80 and 75.16 µg/ml,
compounds
1(3-hexen-1-yl benzoate) and 4 (1-(2,4,5 trihydroxyphenyl)-1-butanone)
exhibited moderate cytotoxic activity with the IC50 value of 120.08
and 152.30 µg/ml, compounds
2 (2,3-dihydroxypropanal) and 11 (oleic acid eicosylester) exhibited less
cytotoxic activity with the IC50 value of 187.50 and 208.22 µg/ml (Table-1).  In A549 lung cancer cell line, compounds 6 and
9 exhibited good cytotoxic activity with the IC50 value of 84.46 and
78.60 µg/ml, compounds
1 and 4 exhibited moderate cytotoxic activity with the IC50 value of
107.30µg/ml and 139.01 µg/ml , compounds
2 and 11 exhibited less cytotoxic activity with the IC50 value of
168.48 and 190.50 µg/ml
against  A549 lung cancer cell lines
(Table-2). The effect of isolated compounds on percentage
growth of MCF-7 and A-549 cell line illustrated in Fig. 2 and Fig, 4 depicts
the cytotoxicity of the isolated compounds at different concentrations of 10,
50, 100, 150 and 200 µg/ml against breast and lung cancer cells. The
results clearly indicate that the compounds 6 and 9 decreases cell viability
and induces the cytotoxic effect in breast cancer MCF-7 cells and lung cancer
A-549 cells more efficiently compared to the other isolated compounds.

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